e faecalis atcc 14428 Search Results


atcc 14428  (ATCC)
93
ATCC atcc 14428
Atcc 14428, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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icg-001  (MedChemExpress)
95
MedChemExpress icg-001
Icg 001, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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setdb2  (Proteintech)
93
Proteintech setdb2
The primers of select genes.
Setdb2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-k8 (ts1  (Thermo Fisher)
90
Thermo Fisher anti-k8 (ts1
The primers of select genes.
Anti K8 (Ts1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hplc daicel chiralpak ad  (Daicel Corporation)
90
Daicel Corporation hplc daicel chiralpak ad
The primers of select genes.
Hplc Daicel Chiralpak Ad, supplied by Daicel Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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eukaryotic translation elongation factor-1α (ef1a  (Thermo Fisher)
90
Thermo Fisher eukaryotic translation elongation factor-1α (ef1a
The primers of select genes.
Eukaryotic Translation Elongation Factor 1α (Ef1a, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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enterococcus faecalis  (ATCC)
98
ATCC enterococcus faecalis
The primers of select genes.
Enterococcus Faecalis, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The primers of select genes.

Journal: Frontiers in Oncology

Article Title: Identifying key transcription factors and immune infiltration in non-small-cell lung cancer using weighted correlation network and Cox regression analyses

doi: 10.3389/fonc.2023.1112020

Figure Lengend Snippet: The primers of select genes.

Article Snippet: Antibodies used in this study included Vimentin (#5741), SLUG (#9585), β-catenin (#8480), and Anti-rabbit Alexa Fluor ® 594 Conjugate (#8889) from Cell Signaling Technology and MMP9 (10375-2-AP), SNAI1 (13099-1-AP), SNAI3 (21350-1-AP), and SETDB2 (14428-1-AP) from Proteintech.

Techniques:

Expression profile and survival analysis of 5 transcription factors (TFs) in NSCLC. (A) Expression profile of 5 TFs (SETDB2, SNAI3, SCML4, ZNF540, and ETV1) in LUAD. (B) Expression profile of 5 TFs (SETDB2, SNAI3, SCML4, ZNF540, and ETV1) in LUSC. (C) Kaplan–Meier analysis of 5 TFs (SETDB2, SNAI3, SCML4, ZNF540, and ETV1) using Affymetrix microarray data. (D) Kaplan–Meier analysis of 5 TFs (SETDB2, SNAI3, SCML4, ZNF540, and ETV1) using RNA-seq data. (E) Analysis of the 5-TFs protein interaction network.

Journal: Frontiers in Oncology

Article Title: Identifying key transcription factors and immune infiltration in non-small-cell lung cancer using weighted correlation network and Cox regression analyses

doi: 10.3389/fonc.2023.1112020

Figure Lengend Snippet: Expression profile and survival analysis of 5 transcription factors (TFs) in NSCLC. (A) Expression profile of 5 TFs (SETDB2, SNAI3, SCML4, ZNF540, and ETV1) in LUAD. (B) Expression profile of 5 TFs (SETDB2, SNAI3, SCML4, ZNF540, and ETV1) in LUSC. (C) Kaplan–Meier analysis of 5 TFs (SETDB2, SNAI3, SCML4, ZNF540, and ETV1) using Affymetrix microarray data. (D) Kaplan–Meier analysis of 5 TFs (SETDB2, SNAI3, SCML4, ZNF540, and ETV1) using RNA-seq data. (E) Analysis of the 5-TFs protein interaction network.

Article Snippet: Antibodies used in this study included Vimentin (#5741), SLUG (#9585), β-catenin (#8480), and Anti-rabbit Alexa Fluor ® 594 Conjugate (#8889) from Cell Signaling Technology and MMP9 (10375-2-AP), SNAI1 (13099-1-AP), SNAI3 (21350-1-AP), and SETDB2 (14428-1-AP) from Proteintech.

Techniques: Expressing, Microarray, RNA Sequencing

Validating the TFs phenotypes, functions, and expression in lung cancer. (A) SETDB2, SNAI3, SCML4, ZNF540, and ETV1 mRNA expression levels in the normal human bronchial epithelial cell line (BEAS-2B), lung adenocarcinoma cell lines (A549, HCC827, H1299, and H1975), and lung squamous cell lines (EPLC-272H, H226, H157, and H2170) using RT-qPCR. Blue box: normal human bronchial epithelial cell line (BEAS-2B). Red box: lung cancer cell lines. (B) The mRNA expression levels of SETDB2 and SNAI3 in the normal human bronchial epithelial cell line (BEAS-2B), lung adenocarcinoma cell lines (A549, HCC827, H1299, and H1975), and lung squamous cell lines (EPLC-272H, H226, H157, and H2170) using RT-qPCR. (C) EdU assays were performed in A549 cells infected with siCtrl, siSETDB2, and siSNAI3. siCtrl, siControl; siSE2, siSETDB2; siSN3, siSNAI3. (D) Wound healing experiments were performed in A549, HCC827, and H1299 transfected with siCtrl, siSETDB2, and siSNAI3. siCtrl, siControl; siSE2, siSETDB2; siSN3, siSNAI3. (E) Transwell invasion assays were performed on lung cancer cells (A549, HCC827, and H1299) infected with siCtrl, siSETDB2, and siSNAI3. siCtrl, siControl; siSE2, siSETDB2; siSN3, siSNAI3. (F) Immunofluorescence staining including MMP9, Vimentin, β-catenin, SNAI1 and SLUG was performed in A549 cells transfected with siCtrl, siSETDB2 and siSNAI3. Blue fluorescence of DAPI labeled cell nucleus, red fluorescence of labeled corresponding protein. siCtrl, siControl; siSE2, siSETDB2; siSN3, siSNAI3. MFI, mean fluorescence intensity. (G) The mRNA expression levels of metastatic markers (MMP9, Vimentin, β-catenin, SNAI1, and SLUG) as well as SNAI3 and SETDB2 were detected by RT-qPCR in A549 cells transfected with siCtrl, siSETDB2, and siSNAI3. siCtrl, siControl. (H) The protein levels of metastatic markers (MMP9, Vimentin, β-catenin, SNAI1, and SLUG) as well as SNAI3 and SETDB2 were detected by western blotting in A549 cells transfected with siCtrl, siSETDB2, and siSNAI3. siCtrl, siControl; siSE2, siSETDB2; siSN3, siSNAI3. (I) The mRNA expression levels of stem cell markers (SOX2, OCT4, and NANOG) in A549 cells transfected with siCtrl, siSETDB2, and siSNAI3 using RT-qPCR. siCtrl, siControl. (B-E, G, I) Error bar represents mean ± SD of three independent experiments. * p < 0.05, ** p < 0.01. Student’s t-test.

Journal: Frontiers in Oncology

Article Title: Identifying key transcription factors and immune infiltration in non-small-cell lung cancer using weighted correlation network and Cox regression analyses

doi: 10.3389/fonc.2023.1112020

Figure Lengend Snippet: Validating the TFs phenotypes, functions, and expression in lung cancer. (A) SETDB2, SNAI3, SCML4, ZNF540, and ETV1 mRNA expression levels in the normal human bronchial epithelial cell line (BEAS-2B), lung adenocarcinoma cell lines (A549, HCC827, H1299, and H1975), and lung squamous cell lines (EPLC-272H, H226, H157, and H2170) using RT-qPCR. Blue box: normal human bronchial epithelial cell line (BEAS-2B). Red box: lung cancer cell lines. (B) The mRNA expression levels of SETDB2 and SNAI3 in the normal human bronchial epithelial cell line (BEAS-2B), lung adenocarcinoma cell lines (A549, HCC827, H1299, and H1975), and lung squamous cell lines (EPLC-272H, H226, H157, and H2170) using RT-qPCR. (C) EdU assays were performed in A549 cells infected with siCtrl, siSETDB2, and siSNAI3. siCtrl, siControl; siSE2, siSETDB2; siSN3, siSNAI3. (D) Wound healing experiments were performed in A549, HCC827, and H1299 transfected with siCtrl, siSETDB2, and siSNAI3. siCtrl, siControl; siSE2, siSETDB2; siSN3, siSNAI3. (E) Transwell invasion assays were performed on lung cancer cells (A549, HCC827, and H1299) infected with siCtrl, siSETDB2, and siSNAI3. siCtrl, siControl; siSE2, siSETDB2; siSN3, siSNAI3. (F) Immunofluorescence staining including MMP9, Vimentin, β-catenin, SNAI1 and SLUG was performed in A549 cells transfected with siCtrl, siSETDB2 and siSNAI3. Blue fluorescence of DAPI labeled cell nucleus, red fluorescence of labeled corresponding protein. siCtrl, siControl; siSE2, siSETDB2; siSN3, siSNAI3. MFI, mean fluorescence intensity. (G) The mRNA expression levels of metastatic markers (MMP9, Vimentin, β-catenin, SNAI1, and SLUG) as well as SNAI3 and SETDB2 were detected by RT-qPCR in A549 cells transfected with siCtrl, siSETDB2, and siSNAI3. siCtrl, siControl. (H) The protein levels of metastatic markers (MMP9, Vimentin, β-catenin, SNAI1, and SLUG) as well as SNAI3 and SETDB2 were detected by western blotting in A549 cells transfected with siCtrl, siSETDB2, and siSNAI3. siCtrl, siControl; siSE2, siSETDB2; siSN3, siSNAI3. (I) The mRNA expression levels of stem cell markers (SOX2, OCT4, and NANOG) in A549 cells transfected with siCtrl, siSETDB2, and siSNAI3 using RT-qPCR. siCtrl, siControl. (B-E, G, I) Error bar represents mean ± SD of three independent experiments. * p < 0.05, ** p < 0.01. Student’s t-test.

Article Snippet: Antibodies used in this study included Vimentin (#5741), SLUG (#9585), β-catenin (#8480), and Anti-rabbit Alexa Fluor ® 594 Conjugate (#8889) from Cell Signaling Technology and MMP9 (10375-2-AP), SNAI1 (13099-1-AP), SNAI3 (21350-1-AP), and SETDB2 (14428-1-AP) from Proteintech.

Techniques: Expressing, Quantitative RT-PCR, Infection, Transfection, Immunofluorescence, Staining, Fluorescence, Labeling, Western Blot